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https://xuanyuan.cloud/agents.md
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https://img.shields.io/badge/nextflow-%E2%89%A50.30.0-brightgreen.svg](https://www.nextflow.io/) https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg](http://bioconda.github.io/) https://img.shields.io/badge/singularity-available-7E4C74.svg]([***]
This pipeline can be used to generate "hints" from aligned sequence evidence to annotate a genome de novo.
Hints file is generated from all available evidences (proteins, EST and/or RNA-seq reads).
Gene models are predicted using Augustus with the hints file as extrinsic evidence (optional).
The minimum requirements are a genome file and at least one type of evidence.
A simple test data set can be downloaded https://***/open?id=1VFqLnRJiuj5Vhj2KCOdY58jwxZKkkMVU
Documentation about the pipeline can be found in the docs/ directory or under the links below:
This pipeline was written by Dr. Montserrat Torres (https://github.com/MontseTor) and Dr. Marc Höppner (https://github.com/marchoeppner) at IKMB. The authors gratefully acknowledge inspiration, fruitful discussions and a few useful code snippets from the [***]
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