
pegi3s/sratoolkitThis image allows the usage of the SRA Toolkit suite. Frequently used tools in this suite are:
fastq-dump: convert SRA data into fastq format.fasterq-dump: convert SRA data into fastq format (faster than fastq-dump).prefetch: allows command-line downloading of SRA, dbGaP, and ADSP data.sam-dump: convert SRA data to sam format.sra-pileup: generate pileup statistics on aligned SRA data.vdb-config: display and modify VDB configuration information.vdb-decrypt: decrypt non-SRA dbGaP data ("phenotype data").Additional tools included in this suite are:
abi-dump: convert SRA data into ABI format (csfasta / qual).illumina-dump: convert SRA data into Illumina native formats (qseq, etc.).sff-dump: convert SRA data to sff format.sra-stat: generate statistics about SRA data (quality distribution, etc.).vdb-dump: output the native VDB format of SRA data.vdb-encrypt: encrypt non-SRA dbGaP data ("phenotype data").vdb-validate: validate the integrity of downloaded SRA data.To obtain the help of an application, you just need to run: docker run --rm pegi3s/sratoolkit <sratoolkit-application-name> --help (e.g. docker run --rm pegi3s/sratoolkit fastq-dump --help)
To run an application, you should adapt and run the following command: docker run --rm -v /your/data/dir:/data pegi3s/sratoolkit <sratoolkit-application-name> /data/mydata.sra --outdir /data/outdir
In this command, you should replace:
/your/data/dir to point to the directory that contains the SRA file you want to convert into a FASTQ file.<sratoolkit-application-name> to the name of the SRA Toolkit application you want to use.mydata.sra to the actual name of your input file.outdir to the actual name of your output directory.For instance, to download data SRR6175516 using the fastq-dump application, you should run: docker run --rm -v /your/data/dir:/data pegi3s/sratoolkit fastq-dump SRR6175516 --outdir /data
Note: Depending on DNS restrictions from your firewall institution, you may obtain an error message:
fastq-dump.3.0.1 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to '[***]
In order to solve it, you need to change your DNS Address. Please follow the steps provided here.



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